Countess Charlotte Brabantina of Nassau (Antwerp, 17 September 1580 – Château-Renard, August 1631) was the fifth daughter of William the Silent and his third spouse Charlotte of Bourbon.
Countess Charlotte Brabantina, her sister Elisabeth and their stepmother Louise de Coligny went to Paris in 1593. Elisabeth married Henri de La Tour d'Auvergne, Duke of Bouillon and at that wedding Charlotte Brabantine met the Claude, Duc de Thouars, whom she married in 1598. The Duke died only six years later, leaving Charlotte Brabantina a widow. They had four children:
| Charlotte Brabantina of Nassau | Father: Prince William of Orange (1533–1584) |
Paternal Grandfather: Count William I of Nassau-Dillenburg (1487–1559) |
Paternal Great-grandfather: Count John V of Nassau-Dillenburg (1455–1516) |
| Paternal Great-grandmother: Landgravine Elisabeth of Hesse-Marburg (1466–1523) |
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| Paternal Grandmother: Countess Juliana of Stolberg-Wernigerode (1506–1580) |
Paternal Great-grandfather: Bodo VIII, Count of Stolberg-Wernigerode (1467–1538) |
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| Paternal Great-grandmother: Anna of Epstein-Königstein (1482–1538) |
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| Mother: Charlotte of Bourbon (1547–1582) |
Maternal Grandfather: Louis, Duke of Montpensier (1513–1582) |
Maternal Great-grandfather: Louis, Prince of La Roche-sur-Yon (1473–1520) |
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| Maternal Great-grandmother: Louise de Bourbon, Duchess of Montpensier (1482–1561) |
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| Maternal Grandmother: Jacqueline de Longwy (before 1524-1561) |
Maternal Great-grandfather: Jean IV de Longwy, Seigneur de Givry, Baron de Pagny et Mirebeau |
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| Maternal Great-grandmother: Jeanne d'Angoulême |